Medical Lab Testing: Spot Test
Spot testing evaluates how well materials resist localized staining and how easily stains can be removed. The test applies specific stains (oils, perspiration, disinfectants, etc.), allows them to set, and then assesses removal during laundering or cleaning. For medical manufacturers, strong spot resistance and cleanability ensure surgical gowns, scrubs, drapes, and textiles maintain a clean, professional appearance after exposure to body fluids or chemicals.
Stains from perspiration, blood, or disinfectants can affect the perceived cleanliness and professional image of medical apparel. Applus+ Reliable Analysis already maintains accredited spot and stain resistance testing capabilities, integrated with our colorfastness, immersion, and cleanability programs.
These evaluations support key standards for protective apparel and medical textiles. Manufacturers use spot testing data to qualify materials that resist staining while remaining easy to clean, helping maintain hygiene and appearance. Applus+ Reliable Analysis delivers consistent, reproducible results using equipment already operating daily under our ISO/IEC 17025 accreditation.
Common Spot Test Medical Testing Standards
AATCC 130 Soil Release Oily Stain Release Method
AATCC 130 evaluates the ability of fabrics to release oily stains during laundering. The test applies an oily stain to the fabric, launders it under standard conditions, and rates the remaining stain using a visual scale. It is widely used for surgical gowns, scrubs, and medical textiles to ensure oily soils from body fluids or lubricants can be effectively removed, maintaining a clean appearance and reducing the risk of residual contamination after laundering.
AATCC 15 Colorfastness to Perspiration
AATCC 15 assesses colorfastness to perspiration by treating specimens with artificial perspiration solution, applying pressure, and incubating. Color change and staining on adjacent fabrics are rated using grey scales. It is essential for medical apparel and textiles to ensure colors do not bleed or stain when exposed to sweat or body fluids, preserving professional appearance and preventing cross-staining during use.
ASTM D543 Resistance of Plastics to Chemical Reagents
ASTM D543 exposes plastic specimens to chemical reagents through spot application or immersion and evaluates changes in appearance, including staining or discoloration. The standard measures visual and physical alterations after exposure. It is commonly used for medical plastics and coated components to ensure they resist localized staining from disinfectants, bodily fluids, or other reagents encountered during use or cleaning.
ISO 105-E04 Textiles Color Fastness to Perspiration
ISO 105-E04 determines colorfastness to perspiration by treating specimens with artificial perspiration solution, applying pressure, and incubating. Color change and staining are rated using grey scales. It is particularly relevant for medical apparel and textiles intended for European markets to ensure colors remain stable and do not transfer when exposed to body fluids.
Expert Medical Spot Test Laboratory You Can Trust
Applus+ Reliable Analysis stands ready to support your medical textile spot and stain resistance testing needs. Our ISO/IEC 17025 accredited laboratory already maintains the necessary spotting and laundering simulation capabilities. This permits us to efficiently add these medical standards with only minor validation and training.
Whether you are developing new surgical gowns, validating scrubs and drapes, or qualifying barrier fabrics, our team provides accurate and reliable data backed by decades of materials testing expertise. We invite you to Contact us to discuss how our expanded medical testing capabilities can support your next project or regulatory submission.
Additional Applus+ Reliable Analysis’ Commercial Medical Testing Capabilities
Whether it be material EMC, environmental, fabrication, or durability testing, Applus+ Reliable Analysis has a full lab of test equipment. This permits us to provide short lead times on scheduling. Our team strives to give our customers more time and energy on product development rather than testing.
Below is a list of common medical testing types that Reliable Analysis offers. Don’t see what you are looking for? Contact us, and we will determine the best course of action.





